Multiple Choice
In an Indirect ELISA, the enzyme-linked antibody will attach to:
Table of contents
- 1. Introduction to Biochemistry4h 36m
- What is Biochemistry?7m
- Characteristics of Life12m
- Abiogenesis13m
- Nucleic Acids16m
- Proteins12m
- Carbohydrates8m
- Lipids10m
- Taxonomy10m
- Cell Organelles12m
- Endosymbiotic Theory11m
- Central Dogma22m
- Functional Groups15m
- Chemical Bonds13m
- Organic Chemistry31m
- Entropy17m
- Second Law of Thermodynamics11m
- Equilibrium Constant10m
- Gibbs Free Energy37m
- 2. Water3h 23m
- 3. Amino Acids8h 10m
- Amino Acid Groups8m
- Amino Acid Three Letter Code13m
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- Amino Acid Configuration20m
- Essential Amino Acids14m
- Nonpolar Amino Acids21m
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- Polar Amino Acids16m
- Charged Amino Acids40m
- How to Memorize Amino Acids1h 7m
- Zwitterion33m
- Non-Ionizable Vs. Ionizable R-Groups11m
- Isoelectric Point10m
- Isoelectric Point of Amino Acids with Ionizable R-Groups51m
- Titrations of Amino Acids with Non-Ionizable R-Groups44m
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- Amino Acids and Henderson-Hasselbalch44m
- 4. Protein Structure10h 4m
- Peptide Bond18m
- Primary Structure of Protein31m
- Altering Primary Protein Structure15m
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- Approximating Protein Mass7m
- Peptide Group22m
- Ramachandran Plot26m
- Atypical Ramachandran Plots12m
- Alpha Helix15m
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- Tertiary Structure of Protein16m
- Protein Motifs and Domains23m
- Denaturation14m
- Anfinsen Experiment20m
- Protein Folding34m
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- 2D-Electrophoresis23m
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- Mass Spectrometry12m
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- Overview of Direct Protein Sequencing30m
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- Indirect Protein Sequencing Via Geneomic Analyses24m
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- 12. Biosignaling9h 45m
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- Jak-Stat Signaling25m
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- Summary of Biosignaling13m
- Signaling Defects & Cancer20m
- Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
- Nucleic Acids 19m
- Nucleic Acids 211m
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- Lipids 111m
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- Practice - Nucleic Acids 111m
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- Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
- Biosignaling 19m
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- Glycolysis 17m
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- Fermentation6m
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- Pentose Phosphate Pathway15m
- Practice - Biosignaling13m
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- Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
- Pyruvate Oxidation9m
- Citric Acid Cycle 114m
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- Metabolic Regulation 18m
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- Glycogen Metabolism 16m
- Glycogen Metabolism 28m
- Fatty Acid Oxidation 111m
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- Citric Acid Cycle Practice 17m
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- Citric Acid Cycle Practice 32m
- Glucose and Glycogen Regulation Practice 14m
- Glucose and Glycogen Regulation Practice 26m
- Fatty Acid Oxidation Practice 14m
- Fatty Acid Oxidation Practice 27m
- Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m
- Amino Acid Oxidation 15m
- Amino Acid Oxidation 211m
- Oxidative Phosphorylation 18m
- Oxidative Phosphorylation 210m
- Oxidative Phosphorylation 310m
- Oxidative Phosphorylation 47m
- Photophosphorylation 15m
- Photophosphorylation 29m
- Photophosphorylation 310m
- Practice: Amino Acid Oxidation 12m
- Practice: Amino Acid Oxidation 22m
- Practice: Oxidative Phosphorylation 15m
- Practice: Oxidative Phosphorylation 24m
- Practice: Oxidative Phosphorylation 35m
- Practice: Photophosphorylation 15m
- Practice: Photophosphorylation 21m
8. Protein Function
ELISA
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Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
In an indirect ELISA, why is the secondary antibody typically conjugated to an enzyme (e.g., horseradish peroxidase or alkaline phosphatase)?
A
To immobilize the antigen on the microtiter plate surface via covalent attachment
B
To generate a measurable signal by converting an added substrate into a detectable product, enabling amplification of antigen detection
C
To increase the affinity and specificity of antigen binding by directly recognizing multiple epitopes on the antigen
D
To prevent non-specific binding by blocking unoccupied sites on the plate
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