Biochemistry

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6. Enzymes and Enzyme Kinetics

Km Enzyme

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1

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Km Enzyme

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2
Problem

What is the initial velocity of a reaction when the concentration of substrate is set equal to the Km?

3

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Km Enzyme

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4
Problem

Select the best description of the K m.

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Km Enzyme

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6
Problem

According to the chart below, which one of the following enzymes has the strongest affinity for its substrate?

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Km Enzyme

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8
Problem

Indicate which region of the Enzyme Kinetics plot below best corresponds to each statement.

A) Initial reaction velocity is limited mainly by the [S] present: ______

B) Initial reaction velocity limited mainly by the [E] present: ______

C) The active site of an enzyme is most likely free/unoccupied: ______

D) The active site of an enzyme is most likely occupied by substrate: ______

E) This region includes the points corresponding to Km & ½Vmax: ______

9
Problem

Use the data in the following chart to determine the Km of the enzyme.