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6. Enzymes and Enzyme Kinetics

1

Michaelis-Menten Assumptions

4m

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2

Michaelis-Menten Assumptions

6m

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3

Problem

Which of the following options is not an assumption made in deriving the Michaelis-Menten equation?

A

The value of k_{-2} can be ignored.

B

The rate of ES formation = to rate of P formation.

C

The [E] is smaller than the [S].

D

a, b & c are all correct assumptions.

4

Problem

Michaelis & Menten assumed that the initial reaction for an enzyme catalyzed reaction could be written as shown:

Using this, the rate/velocity of ES-complex breakdown can be expressed by:

A

k_{1} ([E]_{T} – [ES]).

B

k_{1} ([E]_{T} – [ES])[S].

C

k_{2} [ES].

D

k_{-1} [ES] + k_{2} [ES].