Biochemistry

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7. Enzyme Inhibition and Regulation

Inhibition Constant

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concept

Inhibition Constant

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example

Inhibition Constant Example 1

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concept

Inhibition Constant

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Problem

Use the data in the chart below to determine the answer to the following:

A) Rank the enzymes in order of their binding affinity to their substrate (strongest affinity → weakest affinity).

a) A → B → C. 

b) C → A → B. 

c) C → B → A. 

d) B → A → C.

B) Rank the enzymes in order of their binding affinity to the inhibitor (strongest affinity → weakest affinity).

a) A → B → C. 

b) B → C → A. 

c) C → B → A. 

d) B → A → C.

C) Which enzyme would you expect the inhibitor to affect the most? Why?

a) Enzyme A. 

b) Enzyme B. 

c) Enzyme C.

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concept

Inhibition Constant

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Problem

Calculate the Michaelis constant (Km) and the inhibition constant (K I) given the following information:

[E] = 20 mM. [S] = 15 mM. [ES] = 5 mM. [I] = 8 mM. [EI] = 2 mM.