Biochemistry

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5. Protein Techniques

Tandem Mass Spectrometry

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Tandem Mass Spectrometry

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Problem

Tandem mass spectrometry combines which of the following devices?

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Problem

In your tandem mass-spectrometry of a pure protein, you focused a fragment with an m/z of 1,268 through the process & into the second mass-spec and found y-ion peaks of 1,137 and 1,022. The mass in Daltons for the possible relevant amino acids are provided: Y (163), N (114), W (186), D (115), G (57), L (113) and M (131). What is the order of the first two amino acid residues in the 1,268 fragment from N-terminal to C-terminal?