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Biochemistry
Learn the toughest concepts covered in Biochemistry with step-by-step video tutorials and practice problems by world-class tutors
5. Protein Techniques
Tandem Mass Spectrometry
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1
concept
Tandem Mass Spectrometry
11m
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2
Problem
Tandem mass spectrometry combines which of the following devices?
A
Mass spectrometer with HPLC.
B
Mass spectrometer with chromatography.
C
Mass spectrometer with a PMF database.
D
Mass spectrometer with a mass spectrometer.
3
Problem
In your tandem mass-spectrometry of a pure protein, you focused a fragment with an m/z of 1,268 through the process & into the second mass-spec and found y-ion peaks of 1,137 and 1,022. The mass in Daltons for the possible relevant amino acids are provided: Y (163), N (114), W (186), D (115), G (57), L (113) and M (131). What is the order of the first two amino acid residues in the 1,268 fragment from N-terminal to C-terminal?
A
N-M.
B
N-Y.
C
M-D.
D
M-L.
E
D-N.