Biochemistry

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6. Enzymes and Enzyme Kinetics

Michaelis-Menten vs. Lineweaver-Burk Plots

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Michaelis-Menten vs. Lineweaver-Burk Plots

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Problem

Why is it preferable to use a Lineweaver-Burk over a Michaelis-Menten plot when studying enzyme kinetics?

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Problem

You measure V0 of an enzyme at 6 different [S] & plot the data on a Lineweaver-Burk plot. You then determine the line of best fit to the data to visualize the x & y intercepts. Calculate the Vmax & Km of the enzyme. Hint: pay close attention to the indicated units.