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6. Enzymes and Enzyme Kinetics

1

Michaelis-Menten vs. Lineweaver-Burk Plots

9m

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2

Problem

Why is it preferable to use a Lineweaver-Burk over a Michaelis-Menten plot when studying enzyme kinetics?

A

To directly visualize K_{m} & V_{max} on the plot.

B

To plot kinetic data as a hyperbolic curve instead of a line.

C

To obtain a more accurate measure of the V_{0}.

D

To remove terms that cannot be calculated in a typical enzyme kinetics experiment.

E

To get more accurate estimates of K_{m} & V_{max}.

3

Problem

You measure V_{0} of an enzyme at 6 different [S] & plot the data on a Lineweaver-Burk plot. You then determine the line of best fit to the data to visualize the x & y intercepts. Calculate the V_{max} & K_{m} of the enzyme. Hint: pay close attention to the indicated units.