In enzyme-catalyzed reactions, can the same enzyme molecule be used more than once, and why?

• A. No; the enzyme must be resynthesized after each turnover because it is denatured by catalysis.
• B. Yes; the enzyme is not consumed in the overall reaction and is regenerated after releasing product.
• C. Yes, but only if a covalent enzyme-substrate intermediate permanently stores the substrate.
• D. No; the enzyme is irreversibly converted into product during each catalytic cycle.

Answer: (B)