Multiple Choice
The Vmax for an enzyme is 9 mg/min. Calculate the Km if the [S] = 5 mM when the V0 = 3 mg/min.
6. Enzymes and Enzyme Kinetics
Calculating Km
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To determine the Km from a Lineweaver-Burk plot you would:
A
Multiply the reciprocal of the x-axis intercept by -1.
B
Multiply the reciprocal of the y-axis intercept by -1.
C
Take the reciprocal of the x-axis intercept.
D
Take the reciprocal of the y-axis intercept.
Verified step by step guidance1
Understand the Lineweaver-Burk plot: It is a double reciprocal plot used to analyze enzyme kinetics, where 1/[S] (substrate concentration) is plotted on the x-axis and 1/V (reaction velocity) is plotted on the y-axis.
Identify the x-axis intercept on the Lineweaver-Burk plot. This intercept corresponds to -1/Km, where Km is the Michaelis constant.
To find Km, take the reciprocal of the x-axis intercept. This means if the x-axis intercept is -1/Km, then Km = -1/(x-axis intercept).
Recognize that the y-axis intercept on the Lineweaver-Burk plot corresponds to 1/Vmax, where Vmax is the maximum reaction velocity.
Ensure clarity in calculations: When determining Km from the x-axis intercept, focus on the reciprocal relationship and the sign change involved in the calculation.
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