Your lab isolates a new type of motor protein, which is some version of either myosin, kinesin, or dynein but it is unclear exactly what type it is. You hypothesize that the motor protein is NOT myosin. Which of the following is a piece of evidence that would support your hypothesis?

• A. The protein was isolated by adding then purifying actin microfilaments that it was bound to.
• B. The protein was isolated by adding then purifying microtubules that it was bound to.
• C. The protein aggregated in solution.
• D. The protein did not have a vesicle attached to it when purified.

Answer: (B)

Verified step by step guidance

1. Understand the roles of different motor proteins: Myosin typically interacts with actin filaments, while kinesin and dynein interact with microtubules.
2. Identify the key difference in the problem: The hypothesis is that the motor protein is NOT myosin.
3. Evaluate the evidence: If the protein was isolated using actin microfilaments, it suggests an interaction with actin, which is characteristic of myosin.
4. Consider the correct evidence: If the protein was isolated using microtubules, it suggests an interaction with microtubules, which is characteristic of kinesin or dynein, not myosin.
5. Conclude which evidence supports the hypothesis: The evidence that the protein was isolated by adding then purifying microtubules supports the hypothesis that the protein is not myosin.