In the standard biochemical hydrolysis reaction, what products are formed when ATP is hydrolyzed to ADP?

• A. GTP and inorganic phosphate (Pi)
• B. ADP and inorganic phosphate (Pi)
• C. Adenosine and three inorganic phosphates (3 Pi)
• D. AMP and pyrophosphate ($P{P}_i$)

Answer: (B)

Verified step by step guidance

1. Understand that ATP (adenosine triphosphate) hydrolysis involves breaking one of the high-energy phosphate bonds.
2. Recognize that ATP consists of adenosine and three phosphate groups linked together: alpha, beta, and gamma phosphates.
3. In the standard biochemical hydrolysis reaction, the terminal (gamma) phosphate group is cleaved off from ATP.
4. This cleavage produces ADP (adenosine diphosphate), which contains two phosphate groups, and an inorganic phosphate (Pi) molecule.
5. Therefore, the products of ATP hydrolysis are ADP and inorganic phosphate (Pi), not GTP, AMP, or multiple phosphates.