Textbook Question
Some commonly used restriction enzymes are listed in Table 9.1.
a. Indicate which enzymes produce sticky ends.
b. Of what value are sticky ends in making rDNA?
14. DNA Replication
Introduction to DNA Replication
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Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
Which enzyme is primarily responsible for synthesizing the new DNA strand at the bacterial replication fork?
A
DNA polymerase III
B
Helicase
C
Ligase
D
Primase
Verified step by step guidance1
Understand the role of each enzyme listed in the context of bacterial DNA replication.
Recall that Helicase is responsible for unwinding the double-stranded DNA at the replication fork, creating single-stranded templates.
Recognize that Primase synthesizes a short RNA primer to provide a starting point for DNA synthesis.
Know that DNA polymerase III is the main enzyme that adds nucleotides to the growing DNA strand by extending from the RNA primer.
Identify that Ligase is responsible for joining Okazaki fragments on the lagging strand by forming phosphodiester bonds.
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