Microbiology

Learn the toughest concepts covered in Microbiology with step-by-step video tutorials and practice problems by world-class tutors.

17. Biotechnology

Southern Blotting

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concept

Southern Blotting

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Problem

A southern blotting technique is used to detect a specific ______ sequence from a blood or tissue sample.

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Problem

A _____ is a single-stranded DNA molecule used in hybridization reactions to detect the presence of a particular gene in an assortment of DNA fragments.
(Hybridization Reactions:reactions that combine two complementary single-stranded DNA molecules)

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concept

Steps of Southern Blotting

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Problem

A geneticist wants to see if her patient has Gene X. The geneticist takes a blood sample from her patient and prepares a southern blot. How will the geneticist know if her patient possesses Gene X?

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Problem

Place the following steps of Southern Blotting in the correct order.
a) ______:Filter paper is incubated with the labeled DNA probe which anneals to the ssDNA fragments.
b) ______:Analyze gel to determine the presence of the DNA sequence of interest.
c) ______:Separate DNA fragments by size using gel electrophoresis.
d) ______:Denature DNA by soaking gel in a basic solution.
e) ______:Fragment unknown DNA sample(s) using restriction enzymes.

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Problem

By analyzing the Southern Blot results below, which of the samples contains the gene of interest?