Multiple Choice
Which of the following would be the most effective method in preserving bacterial cultures for long-term storage?
10. Dynamics of Microbial Growth
Introduction to Cultivating Microbial Growth
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Which of the following media are commonly used to cultivate fungi?
A
Nutrient broth
B
Sabouraud dextrose agar
C
Eosin methylene blue agar
D
MacConkey agar
Verified step by step guidance1
Step 1: Understand the purpose of each medium listed. Nutrient broth is a general-purpose liquid medium used to grow a wide variety of bacteria, not specifically fungi.
Step 2: Recognize that Sabouraud dextrose agar is a specialized medium designed to cultivate fungi. It has a low pH and high sugar concentration, which favors fungal growth while inhibiting most bacteria.
Step 3: Know that Eosin methylene blue (EMB) agar is a selective and differential medium primarily used to isolate Gram-negative bacteria, especially coliforms, and is not suitable for fungi.
Step 4: Understand that MacConkey agar is also selective for Gram-negative bacteria and differentiates lactose fermenters from non-fermenters; it is not used for fungal cultivation.
Step 5: Conclude that among the options, Sabouraud dextrose agar is the correct medium commonly used to cultivate fungi due to its selective properties.
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