Place the following steps in order for a neutralization test:
a. Incubate cell culture for a few days.
b. Add suspected infecting virus to the patient's serum.
c. Inspect cell culture for viral infection.
d. Extract serum from patient.
e. Mix patient serum with virus and add the mixture to a cell culture.

Verified step by step guidance

1. d. Extract serum from patient.
2. b. Add suspected infecting virus to the patient's serum.
3. e. Mix patient serum with virus and add the mixture to a cell culture.
4. a. Incubate cell culture for a few days.
5. c. Inspect cell culture for viral infection.

Key Concepts

Neutralization Test

The neutralization test is a laboratory method used to determine the presence of specific antibodies in a serum sample. It involves mixing the serum with a virus to see if the antibodies can neutralize the virus's infectivity. This test is crucial for diagnosing viral infections and assessing immune responses.

Cell Culture

Cell culture is a technique used to grow cells in a controlled environment outside of their natural habitat. In the context of a neutralization test, cell cultures are used to observe the effects of viruses on living cells, allowing researchers to assess viral infection and the effectiveness of antibodies present in the serum.

Incubation Period

The incubation period in microbiology refers to the time required for cells or viruses to grow and develop under specific conditions. In a neutralization test, incubating the cell culture allows for the observation of any viral infection that may occur as a result of the interaction between the virus and the patient’s serum.