14. DNA Synthesis

Which of the following attributes of DNA is most crucial to its accurate duplication?

During the replication of DNA, __________.

Which of the following statements about replication origins is/are correct?

At each end of a DNA replication bubble is __________.

In prokaryotes, the rate of elongation during DNA replication is __________ the rate in eukaryotes.

What is the major difference between bacterial chromosomes and eukaryotic chromosomes?

Describe the process of DNA replication: the ingredients needed, the steps in the process, and the final product.

Analyze the following statements about DNA synthesis. Select True or False for each statement. T/F An RNA polymerase is essential for DNA synthesis. T/F Okazaki fragments would be unnecessary if DNA polymerase could synthesize DNA in both the 3'→5' and 5'→3' directions. T/F DNA ligase is used more frequently on the lagging strand than on the leading strand. T/F Toposiomerase is required to separate the two strands of DNA at the replication fork.

A biochemist isolates, purifies, and combines in a test tube a variety of molecules needed for DNA replication. When she adds some DNA to the mixture, replication occurs, but each DNA molecule consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long. What has she probably left out of the mixture? a. DNA polymerase b. DNA ligase c. Okazaki fragments d. primase