Textbook Question
A mixed culture of Escherichia coli and Penicillium chrysogenum is inoculated onto the following culture media. On which medium would you expect each to grow? Why?
a. 0.5% peptone in tap water
b. 10% glucose in tap water
10. Dynamics of Microbial Growth
Introduction to the Types of Culture Media
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Which type of culture media is used to grow cells anaerobically?
A
Complex media.
B
Enrichment media.
C
Reducing media.
D
Selective media.
Verified step by step guidance1
Understand the requirements for anaerobic growth: Anaerobic organisms require environments devoid of oxygen to grow effectively.
Identify the purpose of reducing media: Reducing media contains agents that remove oxygen from the environment, creating conditions suitable for anaerobic growth.
Compare with other media types: Complex media provides nutrients but does not specifically remove oxygen. Enrichment media enhances the growth of specific organisms but may not be anaerobic. Selective media inhibits the growth of certain organisms but does not necessarily create anaerobic conditions.
Recognize the role of reducing agents: Reducing media often contains chemicals like thioglycolate or cysteine that actively reduce oxygen levels.
Conclude that reducing media is specifically designed to support the growth of anaerobic organisms by creating an oxygen-free environment.
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