In this video, we're going to begin our introduction to conjugation. And so first, recall from our previous lesson videos that conjugation is one of the 3 main types of horizontal gene transfer. And so conjugation is really just the direct transfer of DNA between 2 bacterial cells that are making direct cell to cell contact. Now, conjugation is going to require both a donor cell that will be donating the DNA and a recipient cell that will be receiving the DNA from the donor cell. And also, conjugation can either transfer a plasmid or it could transfer part of the donor cell's chromosomal DNA. Now really there are 3 main types of conjugation that we have numbered down below. 1, 2, 3. And so the first main type of conjugation is going to be F+ plasmid conjugation. The second main type of conjugation is going to be HFR cell conjugation, and then the third main type of conjugation is going to be F' plasmid conjugation where this little apostrophe here is stated as a prime. So this is F' conjugation. Now as we move forward in our course, we're going to talk about each of these 3 main types of conjugation in more detail in their own separate videos. But for now, notice that we're showing you an image or a map of our lesson on these three main types of conjugation. Where on the far left, once again, we have F+ plasmid conjugation, then in the middle what we have once again is HFR cell conjugation. And on the far right, what we have is F' cell conjugation. Now, what you'll notice is that within here is, giving you some details of each of these different types of conjugation. But once again, we're gonna talk more about each of these different types of conjugation as we move forward in their own separate videos. So you don't really need to worry too too much about the details that you see in here right now. You can always come back and review this image after we've covered those, more in-depth videos. And so what you'll notice about F+ plasmid conjugation, one of the main features that you should note that we're gonna emphasize as we move forward as well is that the entire F plasmid is going to be transferred from the donor cell to the recipient cell. With HFR cell conjugation, what we'll see is that only some or only part of the integrated F plasmid is going to be transferred along with some of the donor cells' chromosomal DNA is going to be transferred as well. And once again, we'll be able to talk more about HFR cell conjugation later in our course. And then last but not least, over here on the far right, what we have is F' cell conjugation. And in this one, what we see is that, once again, the entire F' plasmid, oh, with integrated donor DNA is going to be transferred. The entire F' plasmid with integrated donor DNA is going to be transferred. And once again, we'll be able to talk more about F' cell conjugation later in our course in a different video. But for now, this here concludes our brief introduction to conjugation and the 3 main types of conjugation, and we'll get to learn more about these 3 main types of conjugation as we move forward in our course. So I'll see you all in our next video.
16. Microbial Genetics
Introduction to Conjugation
16. Microbial Genetics
Introduction to Conjugation - Video Tutorials & Practice Problems
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Introduction to Conjugation
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Problem
ProblemHow is conjugation different from the two other forms of horizontal gene transfer, transformation and transduction?
A
Conjugation directly transfers DNA from donor to recipient cell.
B
Conjugation requires a phage to transfer the DNA between cells.
C
Conjugation requires a donor and a recipient cell.
D
Conjugation allows the recipient cell to take in free DNA from the environment.
E
A and D.
F
B and C.
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