Some light microscopes are able to create 3-dimensional images of the microscopic specimen. Which class(es) of light microscopes are the 3-D microscopes found in?

• A. Bright-field microscopes.
• B. Light microscopes that increase contrast.
• C. Light microscopes that detect fluorescence.
• D. Both A and B.
• E. Both B and C.
• F. Both A and C.

Answer: (E)

Verified step by step guidance

1. Understand the different classes of light microscopes: Bright-field, contrast-enhancing, and fluorescence-detecting microscopes.
2. Bright-field microscopes are the most common type and typically produce 2-dimensional images by transmitting light through the specimen.
3. Contrast-enhancing microscopes, such as phase-contrast and differential interference contrast (DIC) microscopes, can create 3-dimensional images by enhancing the contrast of transparent specimens.
4. Fluorescence microscopes use fluorescent dyes and special lighting to visualize specimens, and some advanced types, like confocal microscopes, can produce 3-dimensional images by scanning the specimen at different depths.
5. Conclude that the classes of light microscopes capable of producing 3-dimensional images include those that increase contrast and those that detect fluorescence, which corresponds to options B and C.