Which of the following is important when running a load through the autoclave?

• A. Placing items in the autoclave without any water
• B. Ensuring that items are loosely packed to allow steam penetration
• C. Using dry heat instead of steam for sterilization
• D. Sealing all containers tightly to prevent steam entry

Answer: (B)

Verified step by step guidance

1. Understand the principle of autoclaving: it uses steam under pressure to sterilize items by killing microorganisms through moist heat.
2. Recognize that steam must penetrate all surfaces of the items being sterilized to ensure effective sterilization.
3. Identify that placing items loosely allows steam to circulate freely around and inside the materials, enhancing penetration.
4. Note that sealing containers tightly or placing items without water can prevent steam from reaching all surfaces, reducing sterilization effectiveness.
5. Conclude that ensuring items are loosely packed is crucial for proper steam penetration and effective sterilization in an autoclave.