In the context of controlling microbial growth, when should sterile gloves be worn?

• A. Only when working with non-pathogenic microorganisms
• B. During procedures that require aseptic technique to prevent contamination of sterile sites or materials
• C. Whenever handling non-sterile laboratory equipment
• D. While cleaning surfaces with disinfectants

Answer: (B)

Verified step by step guidance

1. Understand the purpose of sterile gloves: they are used to maintain aseptic conditions and prevent contamination of sterile sites or materials during microbiological procedures.
2. Identify situations that require aseptic technique, such as inoculating sterile media, handling sterile instruments, or working with cultures where contamination must be avoided.
3. Recognize that sterile gloves are not necessary when working with non-pathogenic microorganisms unless aseptic technique is required, nor are they needed simply for cleaning surfaces or handling non-sterile equipment.
4. Conclude that sterile gloves should be worn specifically during procedures that require aseptic technique to protect both the sample and the environment from contamination.
5. Remember that proper glove use is part of a broader aseptic protocol, which includes sterilizing tools, working near a flame or in a laminar flow hood, and minimizing exposure of sterile materials to the environment.