When performing a dilution in a laboratory setting, understanding the dilution equation is crucial. A 100-fold dilution, represented as a ratio of 100:1, indicates that the total volume of solvent is 100 times greater than the volume of the solution. In this context, the "100" refers to the combined volume of the solvent and the solution, while the "1" refers specifically to the volume of the solution itself.
To clarify, in a 100-fold dilution, the solvent must always exceed the solution. For example, if you consider various options for achieving this dilution:
- Option A, which suggests a solvent volume less than the solution, is incorrect.
- Option B, with a ratio of 90:10, simplifies to a 10:1 dilution, which is not the desired 100-fold dilution.
- Option C, with 99 parts solvent and 1 part solution, totals 100:1, correctly representing a 100-fold dilution.
- Option D, showing a 100:1 ratio, totals 101:1, indicating a 101-fold dilution, which is also incorrect.
- Option E, with a 10:1 ratio, results in an 11-fold dilution, again not meeting the requirement.
In summary, for a successful 100-fold dilution, ensure that the solvent volume is significantly larger than the solution volume, specifically in a 99:1 ratio, to achieve the correct dilution factor. This understanding is essential for accurate laboratory practices.
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