During activation, an amino acid bonds to the ___ end of tRNA through its ___-terminus.

• A. 5’, C
• B. 3’, N
• C. 5’, N
• D. 3’, C

Answer: (D)

Verified step by step guidance

1. Understand the structure of tRNA: tRNA (transfer RNA) has a specific structure with an anticodon loop and an acceptor stem. The acceptor stem is where the amino acid attaches.
2. Identify the ends of tRNA: The tRNA molecule has two ends, the 5' end and the 3' end. The 3' end is the site where amino acids are attached during the process of tRNA charging or activation.
3. Recognize the amino acid structure: Amino acids have two termini, the amino (N) terminus and the carboxyl (C) terminus. During tRNA activation, the carboxyl group of the amino acid is involved in forming a bond.
4. Determine the bonding process: During activation, the carboxyl (C) terminus of the amino acid forms an ester bond with the hydroxyl group at the 3' end of the tRNA. This process is catalyzed by an enzyme called aminoacyl-tRNA synthetase.
5. Conclude the correct answer: Based on the understanding of tRNA structure and amino acid attachment, the amino acid bonds to the 3' end of tRNA through its C-terminus.