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Multiple Choice
In an experiment, what is the primary purpose of adding multiple drops of a solution onto the same spot on a chromatography paper?
A
To ensure the solvent front moves faster
B
To prevent the sample from spreading
C
To increase the concentration of the sample for better detection
D
To reduce the risk of contamination
Verified step by step guidance
1
Understand the principle of chromatography: it separates components based on their movement through a stationary phase (chromatography paper) with a mobile phase (solvent).
Recognize that adding multiple drops of a solution onto the same spot increases the amount of sample deposited at that location.
Know that increasing the sample concentration at the spot enhances the visibility or detectability of the separated components after development.
Realize that adding multiple drops carefully on the same spot prevents the sample from spreading too much, which could cause poor resolution.
Conclude that the primary purpose of adding multiple drops on the same spot is to increase the concentration of the sample for better detection, not to affect solvent speed or contamination risk.