Multiple Choice
In paper chromatography, what is a likely consequence if a student uses a pen to mark the baseline instead of a pencil?
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4. BONUS: Lab Techniques and Procedures
Chromatography
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What is the main purpose of the mobile phase in chromatography?
A
To detect the separated components after the process
B
To chemically react with the analytes to form colored products
C
To act as the solid support for the separation process
D
To transport the sample components through the stationary phase
Verified step by step guidance1
Understand that chromatography is a technique used to separate components of a mixture based on their different interactions with two phases: the stationary phase and the mobile phase.
Recognize that the stationary phase is typically a solid or a viscous liquid that remains fixed in place and interacts with the components of the mixture.
Identify the mobile phase as the phase that moves through or over the stationary phase, carrying the sample components along with it.
Know that the main purpose of the mobile phase is to transport the sample components through the stationary phase, allowing them to separate based on their differing affinities for the stationary phase.
Note that the mobile phase does not chemically react with the analytes to form colored products, nor does it act as the solid support or detect the components; its role is primarily to facilitate movement and separation.
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